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pGBM2
pGBM2
規(guī)格:
貨期:
編號:B231678
品牌:Mingzhoubio

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產(chǎn)品名稱 pGBM2
商品貨號 B231678
Designations pGBM2
Depositors J Geiselmann
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 4.0460000038146970
DESCRIPTION OF VECTOR:
Intact vector size: 4.046
Type of vector: plasmid
Cloning sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI XbaI BamHI SmaI KpnI SacI EcoRI
Construction: pSC101, pUC18 MCS
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): strR/spcR, <-
replicon: pSC101
MCS: HindIII...EcoRI
Vector: pGBM2 (plasmid)
Construction: pSC101, pUC18 MCS
Marker(s):spcR,strR
Construct size (kb): 4.046000003814697
Features: marker(s): strR/spcR
replicon: pSC101
MCS: HindIII...EcoRI
Applications
The vector can be used whenever it is necessary to maintain two plasmids within the same cell.
Comments
Restriction digests of the clone give the following sizes (kb): BglI--2.9, 1.1; AvaII--3.4, 0.6; XbaI--4.0.
The vector can be used whenever it is necessary to maintain two plasmids within the same cell.
Recommendation for verification: XbaI--4.05; SacI--4.05; BglI--2.94, 1.11; AvaII--3.41, 0.64.
ATCC 87497 and 87498 differ in multiple cloning sites.
Media ATCC® Medium 1639: LB medium (ATCC medium 1065) with 50 mcg/ml spectinomycin
Growth Conditions
Temperature: 37.0°C
References

Manen D, et al. A sensitive reporter gene system using bacterial luciferase based on a series of plasmid cloning vectors compatible with derivatives of pBR322. Gene 186: 197-200, 1997. PubMed: 9074496

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