亚洲国产精品一区二区久久hs,精品国产一级在线观看,免 费 成人黄 色 大片,不卡中文一二三区,国产剧情麻豆mv在线观看,久久久久久久久久久精品,久久九九综合,久久久久久91香蕉国产

Size (kb): 6.3710000000000000

Vector: pZJMalpha (plasmid)

Type: expression

Construction: A 656bp fragment of tubulin is cloned into XhoI/HindIII site.

Construct size (kb): 6.4

Features: marker(s): ampR, BleoR
operator: Tet
promoter: T7
replicon: E. coli Ori
MCS: XhoI, XbaI, NdeI, HpaI, HindIII, SfiI
terminator: T7
transcription terminator: Actin3' UTR, Aldolase 3' UTR

vector for studying DNA-protein interactions tubulin, alpha

PCR-amplified gene fragments can be ligated in a single step between two opposing T7 promoters. This vector can integrate into a nontranscribed region of the Trypanosoma brucei genome and establish a stable cell line. Tetracycline induction leads to the production of a dsRNA with concomitant degradation of the target mRNA.

Temperature: 37.0°C