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45.1-p33-143 [p33-143]
45.1-p33-143 [p33-143]
規(guī)格:
貨期:
編號(hào):B229761
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 45.1-p33-143 [p33-143]
商品貨號(hào) B229761
Designations 45.1-p33-143 [p33-143]
GenBank Number

X00497

Species Homo sapiens, human
Depositors EO Long
Vector
Construct size (kb): 4.400000095367432
Insert
DNA: cDNA
Insert lengths(kb): 1.399999976158142
Tissue: hemizygous 45.1 cell line (DR1, DQw1, DPw2)
Gene product: CD74 antigen (invariant polpypeptide of major histocompatibility class II antigen-associated)(invariant chain, HLA-DR associated) [CD74]
Insert Size (kb) 1.400
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): SmaI/BamHI--3.2, 1.4; PstI--2.05, 1.2, 0.90, 0.37; PvuII--4.6; HindIII--2.9, 1.00, 0.80; BglI--2.9, 1.2, 0.58.
The insert contains the following restriction sites (bp from the 5' SmaI site): PstI--290, 1159; PvuII--291; HindIII--310, 1266; BglI--399; NarI--129.
A full-length cDNA clone.
The ends of the SmaI/BamHI fragment are as follows: 5' SmaI site - GGGCGAGCT(G)15ATG......(A)n [approximately 30 bp of SV40]-BamHI - 3'.
References

Tonnelle C, et al. DObeta: a new beta chain gene in HLA-D with a distinct regulation of expression. EMBO J. 4: 2839-2847, 1985. PubMed: 2998758

Sekaly RP, et al. Cell surface expression of class II histocompatibility antigens occurs in the absence of the invariant chain. J. Exp. Med. 164: 1490-1504, 1986. PubMed: 2430042

Genuardi M, Saunders GF. Localization of the HLA class II-associated invariant chain to human chromosome band 5q32. Immunogenetics 28: 53-56, 1988. PubMed: 3132422

Strubin M, et al. Two forms of the Ia antigen-associated invariant chain result from alternative initiations at two in-phase AUGs. Cell 47: 619-625, 1986. PubMed: 3779839

Strubin M, et al. The complete sequence of the mRNA for the HLA-DR-associated invariant chain reveals a polypeptide with an unusual transmembrane polarity. EMBO J. 3: 869-872, 1984. PubMed: 6586420

Eric O Long, personal communication

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