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pNB-1 [pNb-1]
pNB-1 [pNb-1]
規(guī)格:
貨期:
編號(hào):B227842
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pNB-1 [pNb-1]
商品貨號(hào) B227842
Designations pNB-1 [pNb-1]
GenBank Number

K00057

Species Homo sapiens, human
Depositors J Bishop
Vector
Construct size (kb): 5.0
Insert
DNA: genomic
Insert lengths(kb): 1.0
Tissue: Kelly neuroblastoma
Gene product: avian myelocytomatosis viral (v-myc) related oncogene, neuroblastoma derived [MYCN]
Alleles: C1, C1, C2, C2, C3, C3
Insert Size (kb) 1.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--5.3; BamHI/EcoRI--4.3, 1.0; XhoI--5.5; PstI--3.7, 0.9, 0.8; EcoRI--5.5; XhoI--5.5.
Includes part of intron 1 and exon 2; from approx 1188-2186 on Fig. 2, includes Pst I (2), XhoI, SmaI (2), SstI sites.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
References

Stanton LW, et al. Nucleotide sequence of the human n-myc gene. Proc. Natl. Acad. Sci. USA 83: 1772-1776, 1986. PubMed: 2869488

Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081

Schwab M, et al. Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour. Nature 305: 245-248, 1983. PubMed: 6888561

Barbara Weiffenbach, personal communication

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