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pLDLR-2
pLDLR-2
規(guī)格:
貨期:
編號(hào):B227662
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pLDLR-2
商品貨號(hào) B227662
Designations pLDLR-2
Species Homo sapiens, human
Depositors DW Russell
Applications
in another host, produces protein low density lipoprotein receptor (familial hypercholesterolemia) low density lipoprotein receptor
Vector
Construct size (kb): 8.0
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: LDLR
Genomic copy number: unique
Gene name: low density lipoprotein receptor (familial
hypercholesterolemia)
Contains complete coding sequence?: U
Chromosome: 19; Localization: 19 p13.2
Tissue: fetal adrenal gland
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 5.3
Cross references:
Insert lengths(kb): 5.300000190734863
Tissue: fetal adrenal gland
Gene product: low density lipoprotein receptor (familial hypercholesterolemia)(low density lipoprotein receptor) [LDLR]
Alleles: A2, E1, E2, A1, A2, B1, B2, D1, D2, K1, K2, Q1, Q2, S2, A1, S1
Target Gene: low density lipoprotein receptor (familial hypercholesterolemia)
Insert Size (kb) 5.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--3.0, 1.8, 1.4, 1.1, 0.8; xbaI--uncut; BamHI--3.0, 1.9, 1.7, 1.4, 0.32; HindIII--8.0; EcoRI--8.0.
Encodes nt 1-2580 of the published sequence, including the entire coding region and 3' untranslated region and a portion of the 5' untranslated region.
When pLDLR-2 is transfected into COS-M6 cells, protein is expressed that binds low-density lipoprotein and that is recognized by monoclonal antibody IgG-C7, specific to the human receptor.
The vector contains a portion of pBR322 and SV40 sequences including SV40 ori, 16S and 19S donor and acceptor splicing sites, and polyadenylation signals.
Constructed by joining the cDNA insert of p203 (nt 1-323) and p101 (nt 324-5144) via overlapping HgiAI sites.
References

Yamamoto T, et al. The human LDL receptor: a cysteine-rich protein with multiple Alu sequences in its mRNA. Cell 39: 27-38, 1984. PubMed: 6091915

Brown MS, et al. Methods and compositions for the detection of familial hypercholesterolemia. US Patent 4,966,837 dated Oct 30 1990

Sudhof TC, et al. The LDL receptor gene: a mosaic of exons shared with different proteins. Science 228: 815-822, 1985. PubMed: 2988123

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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