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pCN41
pCN41
規(guī)格:
貨期:
編號(hào):B227642
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCN41
商品貨號(hào) B227642
Designations pCN41
Depositors R Diaz Orejas
Biosafety Level 1
Vector Information
Size (kb): 4.5000000000000000
Vector: pCN41 (plasmid)
Construction: pCN40
Marker(s):strR
Construct size (kb): 4.5
Features: marker(s): strR
replicon: repA
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.4, 1.1, 1.0; KpnI/SmaI--4.6; PvuII/PstI--2.5, 2.0; KpnI--4.6; SmaI--uncut.
Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences.
General purpose vector.
Constructed by excising a BamHI/HindIII fragment encoding part of kanR from pCN40 (ATCC 77097), blunting with Klenow fragment, and religating.
Media ATCC Medium 1065 (see below) plus streptomycin ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References

Nieto C, et al. Cloning vectors, derived from a naturally occurring plasmid of Pseudomonas savastanoi, specifically tailored for genetic manipulations in Pseudomonas. Gene 87: 145-149, 1990. PubMed: 2110095

Ramon Diaz Orejas, personal communication

Shipped freeze-dried
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周經(jīng)理 17757487661 1296385441
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沈經(jīng)理 19548299266 2662369050
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