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KBP5
KBP5
規(guī)格:
貨期:
編號:B227352
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 KBP5
商品貨號 B227352
Designations KBP5
GenBank Number

U07000

Species Homo sapiens, human
Depositors S Clifton, BA Roe
Vector
Construct size (kb): 48.40000152587891
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: svPHEP
Intact vector size: 9.201
Type of vector: cosmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI ClaI
Polylinker sites: HindIII ClaI EcoRI BamHI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1
Cross references: DNA Seq. Acc.: L19900
Insert
DNA: genomic
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: BCR
Other gene symbols: BCR1
Genomic copy number: unique
Gene name: breakpoint cluster region
Contains complete coding sequence?: N
Chromosome: 22; Localization: 22 q11
Tissue: chronic myelogenous leukemia K-562 cell line
Type of DNA: genomic
Insert end: MboI
Insert end: MboI
Insert size (kb): 39.199
Cross references: DNA Seq. Acc.: U07000
Nucleotides 1-39199 of the insert correspond to
nucleotides 85435-124633 of U07000.
Insert lengths(kb): 39.19900131225586
Tissue: chronic myelogenous leukemia K-562 cell line
Gene product: breakpoint cluster region [BCR]
Alleles: A1, A1, A2, A2
Insert Size (kb) 39.199
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 12.5, 10.5, 7.0, 1.45, 0.52; BglII--13.5, 9.8, 7.0, 6.2, 4.8, 2.7, 2.6, 1.75, 0.92, 0.54; EcoRI--20.0, 17.0, 6.6, 2.75, 1.2, 1.1, 0.6;
SstI--7.5, 6.4, 4.4, 3.8, 3.5 (doublet), 3.35, 2.15, 2.05, 1.7, 1.45, 1.3, 0.62, 0.4; XbaI--18.0 (doublet), 10.5, 2.35; SalI--33.0, 16.0.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
The insert begins in intron 3' and continues into exon 15. This region includes an open reading frame (96845-97681) that appears to be expressed.
References

Grosveld G, et al. The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript. Mol. Cell. Biol. 6: 607-609, 1986. PubMed: 3023859

Chissoe SL, et al. Sequence and analysis of the human ABL gene, the BCR gene, and regions involved in the Philadelphia chromosomal translocation. Genomics 27: 67-82, 1995. PubMed: 7665185

Bruce A Roe, personal communication

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