亚洲国产精品一区二区久久hs,精品国产一级在线观看,免 费 成人黄 色 大片,不卡中文一二三区,国产剧情麻豆mv在线观看,久久久久久久久久久精品,久久九九综合,久久久久久91香蕉国产

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Trypanosoma brucei Plimmer and Bradford
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Trypanosoma brucei Plimmer and Bradford
Trypanosoma brucei Plimmer and Bradford
規(guī)格:
貨期:
編號:B227329
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Trypanosoma brucei Plimmer and Bradford
商品貨號 B227329
Strain Designations Lister 427 procyclic form
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation Unknown; possibly derived from s427 strain, Uganda, 1960
Product Format frozen
Storage Conditions Frozen: -70°C or colder
Live Culture: See Protocols Section
Comments Wild type procyclic form
Medium ATCC® Medium 2831: SDM-79 Medium
ATCC® Medium 431: Trypanosome medium
Growth Conditions Temperature: 27°C
Cryopreservation Harvest and Preservation
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 0.5–1.0 x 107/mL in fresh growth medium.  If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 20% (v/v) solution of sterile glycerol in fresh growth medium. 
  4. Mix the cell preparation and the glycerol solution in equal portions. The final concentration will be 2.5-5 x 106 cells/mL in 10% glycerol. The time from the mixing of the cell preparation and glycerol stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials.
  6. Place the ampules in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.  (The cooling rate in this apparatus is approximately -1°C/min.)  If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.  At -40°C, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.  Do not agitate the ampule.  Do not leave ampule in water bath after it is thawed.
  9. Remove the vial from the water bath immediately after thawing.  Aseptically transfer the contents of the ampule into 10 mL of fresh growth medium. 
  10. Incubate the flask at 27°C with the cap screwed on tightly.
  11. Maintain as described above. 
Name of Depositor G Cross
References

Wirtz E, et al. A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei. Mol. Biochem. Parasitol. 99(1): 89-101, 1999. PubMed: 10215027

Cunningham MP, Vickerman K. Antigenic analysis in the Trypanosoma brucei group, using the agglutination reaction. Trans. R. Soc. Trop. Med. Hyg. 56: 48-59, 1962. PubMed: 13882652

Cross GA, Manning JC. Cultivation of Trypanosoma brucei sspp. in semi-defined and defined media. Parasitology 67(3): 315-331, 1973. PubMed: 4761771

Peacock L, et al. Fly transmission and mating of Trypanosoma brucei brucei strain 427. Mol Biochem Parasitol 160(2): 100-106, 2008. PubMed: 18524395

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
蓬莱市| 义乌市| 凤山市| 罗田县| 运城市| 朝阳市| 新龙县| 邓州市| 遂宁市| 合川市| 山阴县| 伽师县| 迁安市| 中阳县| 大冶市| 永福县| 明光市| 万年县| 文安县| 凤翔县| 靖宇县| 南岸区| 文化| 齐齐哈尔市| 德惠市| 城口县| 长垣县| 建阳市| 湖州市| 辛集市| 图片| 钟祥市| 九龙县| 蓝田县| 临汾市| 洛浦县| 葵青区| 丰城市| 凌海市| 什邡市| 东乌|