| 產(chǎn)品名稱(chēng) |
Monkey cytomegalovirus |
| 商品貨號(hào) |
B236761 |
| Classification |
Herpesviridae, Cytomegalovirus |
| Agent |
Monkey cytomegalovirus |
| Strain |
68-1 |
| Common Name |
African green monkey cytomegalovirus |
| Applications |
Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM). |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Urine of rhesus monkey, 1968 |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder
(or colder) |
| Comments |
Sensitive to heat (exposure to 56C for 10 min. completely inactivated 10(4) TCID[50] of Rhesus CMV). Produces enlarged, distorted nuclei with bizarre inclusions infected human and monkey fibroblast TC. Has morphology of the herpes group (by EM). |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects (refractile rounding and sloughing) CPE, refractile rounding and sloughing |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host |
Recommended Host:? MRC-5 (ATCC CCL-171) |
| Growth Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 35.0°C Duration: 3 to 30 days; For best results, cells should be 24 to 48 hours old and 70-80% confluent (not 100% confluent) at inoculation. |
| Cryopreservation |
Storage temperature: -70°C |
| Name of Depositor |
DM Asher |
| Source |
Urine of rhesus monkey, 1968 |
| Year of Origin |
1968 |
| References |
Kaur A, et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed: 8892893
Asher DM. . Bacteriol. Proc. 269: 91, 1969.
Asher DM, et al. Persistent shedding of cytomegalovirus in the urine of healthy Rhesus monkeys. Proc. Soc. Exp. Biol. Med. 145: 794-801, 1974. PubMed: 4131945
or colder
almost any human or monkey diploid fibroblast tissue culture cells may be used
or colder
|
